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Make a Dichotomous Key using the folowing bacteria to ultimately isolate the fol

ID: 191140 • Letter: M

Question

Make a Dichotomous Key using the folowing bacteria to ultimately isolate the following organims using PEA, gram stain, catalase test, oxidase test, MSA, etc.:

Proteus                           Acinetobacter                       Staphylococcus                     Micrococcus

Lactobacillus                  Salmonella                            Escherichia                            Neisseria

Bacillus                          Shingella                               Klebsiella                              Citrobacter

Serratia                           Enterobacter                         Pseudomonas                        Streptococcus

Explanation / Answer

Bacteria

PEA

Gram Stain

Catalase Test

Oxidase Test

Proteus

Inhibits the growth

Negative

Positive

Negative

Lactobacilllus

Promotes the growth

Positive

Negative

Negative

Bacillus

Promotes the growth

Positive

Positive

Negative

Serratia

Inhibits the growth

Negative

Positive

Negative

Acinetobacter

Inhibits the growth

Negative

Positive

Negative

Salmonella

Inhibits the growth

Negative

Positive

Negative

Shingella

Inhibits the growth

Negative

Positive

Negative

Enterobacter

Inhibits the growth

Negative

Positive

Negative

Staphylococcus

Promotes the growth

Positive

Positive

Negative

Escherichia

Inhibits the growth

Negative

Positive

Negative

Klebsiella

Inhibits the growth

Negative

Positive

Negative

Pseudomonas

Inhibits the growth

Negative

Positive

Negative

Micrococcus

Promotes the growth

Positive

Positive

Positive

Neisseria

Inhibits the growth

Negative

Positive

Positive

Citrobacter

Inhibits the growth

Negative

Positive

Negative

Streptococcus

Promotes the growth

Positive

Negative

Negative

PEA Test – Phenylethyl alcohol agar (PEA) is a selective medium test which is specific for gram positive bacteria. The selective medium inhibits the growth of gram negative bacteria and promotes growth of gram positive ones.

Bacteria

PEA

Gram Stain

Catalase Test

Oxidase Test

Proteus

Inhibits the growth

Negative

Positive

Negative

Lactobacilllus

Promotes the growth

Positive

Negative

Negative

Bacillus

Promotes the growth

Positive

Positive

Negative

Serratia

Inhibits the growth

Negative

Positive

Negative

Acinetobacter

Inhibits the growth

Negative

Positive

Negative

Salmonella

Inhibits the growth

Negative

Positive

Negative

Shingella

Inhibits the growth

Negative

Positive

Negative

Enterobacter

Inhibits the growth

Negative

Positive

Negative

Staphylococcus

Promotes the growth

Positive

Positive

Negative

Escherichia

Inhibits the growth

Negative

Positive

Negative

Klebsiella

Inhibits the growth

Negative

Positive

Negative

Pseudomonas

Inhibits the growth

Negative

Positive

Negative

Micrococcus

Promotes the growth

Positive

Positive

Positive

Neisseria

Inhibits the growth

Negative

Positive

Positive

Citrobacter

Inhibits the growth

Negative

Positive

Negative

Streptococcus

Promotes the growth

Positive

Negative

Negative

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