Describe the essential activities of each of the following enzymes in DNA replic

Question

Describe the essential activities of each of the following enzymes in DNA replication (12 pts).

A. DNA polymerase I:

a.______________________________________________________

b.______________________________________________________

c. ______________________________________________________

B. DNA polymerase III:

a.______________________________________________________

b.______________________________________________________

C. Primase: __________________________________________________________________

Explanation / Answer

Ans:

The essential activities of the following enzymes in DNA replication:

A. DNA polymerase I:

a. DNA polymerase I processes RNA primers during lagging strand synthesis and fills small gaps during DNA repair reactions.

b. DNA polymerase I is mainly responsible for the synthesis of new strand of DNA.

c. DNA polymerase I has 3' to 5' exonuclease activity which enables it to proofread the newly synthesized DNA strand and thereby, correct errors made during DNA replication.

B. DNA polymerase III:

a. It is essential for DNA synthesis.

b. DNA polymerase III extends RNA primers on both strands.

C. Primase:

A primase is an enzyme which makes the RNA primer required for the initiation of Okazaki fragments on the lagging strand.

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