Can someone help me figure out how to solve this question? Would it travel the s

Question

Can someone help me figure out how to solve this question? Would it travel the same distance?

A LHON mutation in another NADH dehydrogenase subunit gene causes the loss of a Mae Ill restriction enzyme site. Compared to normal DNA digested with Mae III, the DNA from a patient with LHON digested with Mae Ill would be expected to A) Travel a shorter distance during gel electrophoresis BTravel a greater distance during gel electrophoresis Travel the same distance during gel electrophoresis D) Travel the same distance as it would if it were not treated with the Mae IlIl restriction enzyme

Explanation / Answer

Answer: A: Travel a shorter distance during the gel elctrophoresis

Explanation: As there ccurs a mutation in NADH dehydrogenase gene that causes loss of Mae II restriction enzyme site, therefore at this position DNA will not cut. In this way it will remain a larger DNA fragment compared to what will happen if it is digested. In conclusion, we will have larger DNA fragment due to loss of restriction site. As we know the migration of a DNA fragment on the agarose gel is inversley propotional to size of DNA fragemt. Therefore, the larger fragment will now move slower.

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