Next, obtain reagents for your standard curve by mixing BSA samples differing in
ID: 191462 • Letter: N
Question
Next, obtain reagents for your standard curve by mixing BSA samples differing in protein concentration (a stock of BSA has been prepared for you at a concentration of 2 mg/ml). To do this, label a set of microfuge tubes from 1-6. Then, follow the chart below to fill the tubes with appropriate amounts of BSA and water. Be sure to thoroughly mix tubes. Determine the final BSA concentration using the equation C1V1 = C2V2
Tube Volume of Water Volume of BSA (Stock) Final BSA Concentration
1 10 ul 10 ul ____ ug/ml
2 12.5 ul 7.5 ul ____ug/ml
3 15 ul 5 ul ____ug/ml
4 17.5 ul 2.5 ul ____g/ml
5 19 ul 1.0 ul ____ug/ml
6 20 ul 0uL 0 ug/ml (blank)
Explanation / Answer
C1 = 2 mg/ml = 2000 ug/ml
V1 = Column 2 (Volume of BSA (Stock))
V2 = Column 1 (Volume of Water) + Column 2 (Volume of BSA (Stock))
C2 = C1 V1/V2
Tube Volume of Water Volume of BSA (Stock) Final BSA Concentration
1 10 ul 10 ul 1000 ug/ml
2 12.5 ul 7.5 ul 750 ug/ml
3 15 ul 5 ul 500 ug/ml
4 17.5 ul 2.5 ul 250 ug/ml
5 19 ul 1.0 ul 100 ug/ml
6 20 ul 0 uL 0 ug/ml (blank)
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