#2 Imagine you do several experiments in which you examine the effects of differ
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#2 Imagine you do several experiments in which you examine the effects of different antibiotics on translation. For each experiment, you use set up a cell-free in vitro translation system (which has all the components needed for translation), to which you add the antibiotic you are testing You hen add the mRNAwith the sequence AUGAAAAAAAAAAAAAAAAAAAAAUAA to the 5' experiment. For each antibiotic treatment, you get the polypeptide shown after translation is allowed to proceed. Based on this data, suggest a possible step in translation that is affected directly by each antibiotic affect? EXPT# ANTIBIOTIC PRODUCT control none STEP AFFECTED* fimet-lys-lys-lys-lys-lys-lys-lys- damycin No polypeptide product made 2 spectinomycin fmet-lys 4 spidermycin Fmet Optional: Give a rationale for each of your answers in #2. 3 erythromycin fmet-lys-lys-lysExplanation / Answer
1. Clindamycine bounds with the 50 s r RNA subunit of the ribosome and do not allow the binding of 30 s ribosomal subunit and does not allow the binding of both ribosomal subunits and leads to inhibition of production of protein.
2. Spectinomycine: It binds to the 30 s subunit of ribosomes and does not allow the continuous synthesis of protein. In ribosomes initiator t RNA comes and bind with the 50 s subunit and then allowd the binding of elongation factor and then after binding of 30 s subunits to the ribosomal 50 s subunit occurs. So this binding is inhibited by this antibiotic. That is why I know continue synthesis of protein.
3. Erythromycin: It binds to the 23 rRNA present in the 50 s subunit and inhibits the continuation of peptide chain elongation.
4. Spidermycin: Binds to the 50 s subunit and block it so no synthesis of protein.
first of all ribosomal subunit 50 s and 30 s subunit dissociates from each other. Then after mRNA do binding to the ribosome and continues the synthesis of protein.
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