is experiment, you will make Protein Standards using a Dilution scheme. Read for

Question

is experiment, you will make Protein Standards using a Dilution scheme. Read form the required calculations. Include your calculated through the directions in the lab and per answers in this prelab and in the lab protocol. (Hint: Use C, V-CV 4a. You will make 10ml of 2mg/ml (or 2000g /ml) of BSA. How much will you weigh (in GRAMS) for 10ml of 2mg/ml? 4b. Second, you will make 10ml of 100 g/ml. How much 2000 /ml BSA will you add to make 10mL of 100 1ml? 4c. Next, you will make 1 ml of 5 g/ml. ul 4d. Next, you will make 1 ml of 10g/ml. ul of water (to make 1 ml total). ulof water (to make 1ml total). ul of water (to make 1 ml total). -ul of water (to make 1 ml total). Add of your 100g/ml BSA and of your 100g/ml BSA and of your 100g/ml BSA and -ul of your 100ginl BSA and Add 4e. Next, you will make 1 ml of 20g/ml. ul 4e. Next, you will make i ml of 30g/ml. Add Add

Explanation / Answer

4a. 0.02g

4b. 0.5 milliroliter (ml)

4c. 50microlit of sol. will be added to 950microli. of water

4d. 100microlit of sol. will be added to 900microlit. Of water

4e. 200microlit of sol. will be added to 800microlit of water

4f. 300microlit of sol. will be added to 700 microlit of water

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