4. (4pts) In this experiment, you will make Protein Standards using a Dilution s

Question

4. (4pts) In this experiment, you will make Protein Standards using a Dilution scheme. Read through the directions in the lab and perform the required calculations. Include your calculated answers in this prelab and in the lab protocol. (Hint: Use C,V 4a. You will make 10ml of 2mg/ml (or 2000g/ml) of BSA. 4b. Second, you will make 10ml of 100g/ml. 4c. Next, you will make 1 ml of 5g/ml. 4d. Next, you will make iml of 10g/ml. How much will you weigh (in GRAMS) for 10mL of 2mg/ml? How much 2000 /ml BSA will you add to make 10mL of 10Oug/ml? Add of your 100g/ml BSA and ul of your 100gl ml BSA and ul of your 100gl ml BSA and ul of your 100g/ml BSA and ul of water (to make 1 ml total). ul of vwater (to make 1 ml total). ul of water (to make 1 ml total). ul of water (to make l ml total). ul Add 4e. Next, you will make 1 ml of 20g/ml. Add 4e. Next, you will make 1ml of 30g/ml. Add 29

Explanation / Answer

4a) To make a solution of 10 ml of 2 mg/ml solution, we have to weigh,

= 2 x 10

= 20 mg

= 0.02 grams

4b) C1V1 = C2V2

(2000)V1 = (100)(10)

V1 = 1000/2000

= 0.5 ml

4c) (1)(5) = V2 (100)

V2 = 0.05 ml

= 50 micro ml of BSA and 950 micro ml of water

4d) (1)(10) = V2 (100)

V2 = 0.1 ml

= 100 micro ml of BSA and 900 micro ml of water

4e) (1)(20) = V2 (100)

V2 = 0.2 ml

= 200 micro ml of BSA and 800 micro ml of water

4f) (1) (30) = V2 (100)

V2 = 0.3 ml

= 300 micro ml of BSA and 700 micro ml of water

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