Problem 3: You form liposomes in vitro that contain transporters and channels. T

Question

Problem 3: You form liposomes in vitro that contain transporters and channels. Then you add the following mixture of solutes to the outside of the liposomes 0.1mM alanine 1mM CaCl2 100mM NaC 1uM testosterone 1mM ATP Which type of channels/transporters need to be present in the liposome to cause import of the solutes into the lumen of the liposome, reaching the final concentrations of (final concentrations of the solutes in the liposome lumen): 1mM alanine 0.01mM Ca2* 1mM Na* 100mM CI 1uM testosterone 1mM ATP

Explanation / Answer

Alanine and ATP enter into the lumen of the Liposome by a special type of protein Transporter. This process is called Facilitated Diffusion. As these two molecules are Charged and large ones.

Ca+2, Na+ and Cl- will enter into Lumen of the Liposome through Channels by Simple Diffusion

Testosterone is a Hydrophobic molecule, which can dissolve in the Liposomal membranes and can enter into Lumen directly by Diffusion across the Liposomal lipid bilayer. It doesn't require any proteins like Transporter or Channel

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