Set up Bovine Serum Albumin dilutions (prepared in 0.01% SDS) to provide a stand
ID: 192395 • Letter: S
Question
Set up Bovine Serum Albumin dilutions (prepared in 0.01% SDS) to provide a standard curve for future lab experiments. Each group will be given 1 mL ampoules of 2 mg/ml BSA. We will have a few extra ampoules in case of accidents. Make your dilutions in 1.5 mL eppendorf tubes.
Final BSA Concentration
Concentration of BSA Stock
Desired Volume
Volume of Stock (to be added)
Volume of 0.01% SDS (to be added)
5ug/100uL
100uL
10ug/100uL
100uL
20ug/100uL
100uL
30ug/100uL
100uL
40ug/100mL
100uL
50ug/100uL
100uL
60ug/100uL
100uL
70ug/100uL
100uL
Final BSA Concentration
Concentration of BSA Stock
Desired Volume
Volume of Stock (to be added)
Volume of 0.01% SDS (to be added)
5ug/100uL
100uL
10ug/100uL
100uL
20ug/100uL
100uL
30ug/100uL
100uL
40ug/100mL
100uL
50ug/100uL
100uL
60ug/100uL
100uL
70ug/100uL
100uL
Explanation / Answer
final BSA conc.
final BSA conc.
Conc. of BSA stock Desired volume Volume of stock (to be added) Volume of 0.01%SDS to be added 5ug/100ul 2000ug/2000ul 100ul 0.25ul 99.75ul 10ug/100ul 2000ug/2000ul 100ul 0.5ul 99.5ul 20ug/100ul 2000ug/2000ul 100ul 1ul 99ul 30ug/100ul 2000ug/2000ul 100ul 1.5ul 98.5ul 40ug/100ul 2000ug/2000ul 100ul 2ul 98ul 50ug/100ul 2000ug/2000ul 100ul 2.5ul 97.5ul 60ug/100ul 2000ug/2000ul 100ul 3ul 97ul 70ug/100ul 2000ug/2000ul 100ul 3.5ul 96.5ulRelated Questions
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