the information in the table provided to answer the problems Chemical Bovine ser
ID: 192530 • Letter: T
Question
the information in the table provided to answer the problems Chemical Bovine serum abumin (BSA) Glucose (CHO) Sodium crioride (N) Socium itrate(Na CH.0) Sodkum oodesyl sutate (SDS, N.tso) Tris( C.HIND) Molecular weight 66.5 KD 180 58.4 258 288 121.1 Remember to SHOW ALL WORK in your answers, include units, and circle your final answer. Be specific when asked how to make a solution, discuss the details in your answer 1. Calaiate how much glucose You need to make soo ml of 5% glucose solution. 2. Calculate the molarity of the glucose solution you made in Qt. 3. How wouid you make 2 Iters of a 0.5 M irts buffier, p 7.6 4. How would you make 500 ml of 5 M Na0? 5. How would you make i liter of 2 M sodium citrate? Maa, 0.3 M sodium ctrate, using the soltions you made in Q4 and Q5 above? 7. Calcuate how much S0S you need to make 800 ml of a 56 SDS solution 8. Calouate how much SDS and SSC you need from the solubons made in Q6 and Q7 to make 1 liter or 0.1x ssc, 0.1% SDS. 9. How would you make 1 tter of 20 mM Tris, 10 met bNaCl from the solutions made in Q3 and 24? 10.How much BSA would you read to add to re solution ngetomake t 1%asExplanation / Answer
Ans-1 to make 5% glucose solution we will need
5/100 × 500 = 25 gms of glucose will be needed to make 500 ml of 5% glucose solution.
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