Quiz (2 min) Western Blotting for Protein-X Wild-type Knockout-X Wild-type Knock

Question

Quiz (2 min) Western Blotting for Protein-X Wild-type Knockout-X Wild-type Knockout-X -200kDa 100kDa 100kDa 50kDa 35kDa Antibody-A Antibody-B Which of the following statement is correct? 1. Protein-X generates multiple forms of polypeptide at 200, 100, 50, 35kDa sizes 2. Antibody-B is superior than Antibody-A since it recognizes only a single band 3. Wild-type protein-X has a molecular weight of about 200 kDa 4. The mutation of gene-X causes an increase in protein degradation 5. Antibody-A is polyclonal and Antibody-B is monoclonal.

Explanation / Answer

By looking at the image Protein X in one band is wild type and another is knockout. So in wild type there should be expression in wild type and there should not be any band in Knockout. So with antibody A at the molecular weight 200kDa the band is missing from Knockout though it is present in the wild type. We can infer that Wild type protein has a molecular weight of abbout 200kDa.

The right answer is 3- Wild type protein-X has a molecular weight of about 200kDa

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