If you purified a novel digestive enzyme and wanted to denature it by disrupting

Question

If you purified a novel digestive enzyme and wanted to denature it by disrupting all tertiary structure, which of the following would be effective? A. Boiling B. A reducing agent like beta-mercaptoethanol (BME) C. A mutation that alters a single amino acid in the protein D. None of the above If you purified a novel digestive enzyme and wanted to denature it by disrupting all tertiary structure, which of the following would be effective? A. Boiling B. A reducing agent like beta-mercaptoethanol (BME) C. A mutation that alters a single amino acid in the protein D. None of the above A. Boiling B. A reducing agent like beta-mercaptoethanol (BME) C. A mutation that alters a single amino acid in the protein D. None of the above

Explanation / Answer

Answer: B. A reducing agent like beta-mercaptoethanol (BME)

Explanation: Beta-mercaptoethanol (MBE) cleaves the disulfide bonds and thereby, proteins can be denatured.

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.