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you and your lab partner are each asked to transform a different bacterium. you

ID: 192820 • Letter: Y

Question

you and your lab partner are each asked to transform a different bacterium. you perform a control with the organisms you used in today's lab, while she uses an organism she isolated from some pond water. your transformation worked perfectly, but hers did not. how could you explain this result?
a) you did not incubate the plates long enough to see growth
b) the pond water denatured the DNA before the bacterium could incorporate it
c) the DNA you both were using was ineffective
d) the bacteria from the pond water were not competent.

Explanation / Answer

d) the bacteria from the pond water were not competent.

Some bacteria can take foreign extracellular DNA , which is called copetent bacteria. Bacterial competence is necessary for transformation procedure.