3.(2) In 3 transduction experiments, here are the donor cell and the recipient c

Question

3.(2) In 3 transduction experiments, here are the donor cell and the recipient cell (each row of the table is a separate expt). For each row, you select for a marker as shown (remember that select means to kill off everything except the cells that have that marker) and among those that live, you ask if they contain or do not contain the unselected marker. The last column shows the % of cells that contain the unselected marker. From these data, figure out the order of the genes and which two are closest together. Hint: there are only 3 possible orders. Donor Recipient Selected marker unselected marker% Et Gteg- E+ G+H+g-H H+ E+ H+e-h H+ the g gene the g gene the e gene 26% 54% 5%

Explanation / Answer

From the observations,it is seen that,

No.of G+ cells/ Total no.of E+ cells=26/100

No.of G+ cells/ Total no.of H+ cells=54/100

No.of E+ cells/ Total no.of H+ cells=5/100

So,it can be seen that co-transduction frequency between G+ and H+ is very high and between E+ and H+ is very low.

So G+ and H+ genes are closest together and the order of the genes is= E+ G+ H+

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