ringGenetics: 4. Restriction enzymes and vectors-Opera eringgenetics.com/myct/it
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ringGenetics: 4. Restriction enzymes and vectors-Opera eringgenetics.com/myct/itemView Restriction enzymes and vectors Question Restriction enzyme(s) Bar and Hael BamHl ony 1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? EcoRi only BamHl and Hind 2. Which enzyme(s) would cut the plasmid without disrupting the function of the amp gene? BamHl. EcoR and Haell Hoell ony 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Hindlli only 4. Which one restriction enzyme satisfies all three BamHi and of the requirements listed above? and HindiExplanation / Answer
1) BamHI, HaeIII, and HindIII enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene.
2) BamHI, EcoRI, and HaeIII enzyme(s) would cut the plasmid without disrupting the function of the ampR gene.
3) BamHI, EcoRI, and HindIII enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid.
4) BamHI only one restriction enzyme satisfies all three of the requirements listed above.
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