My doubts are in the C and D A biochemist studies the properties of a metabolic

Question

My doubts are in the C and D

A biochemist studies the properties of a metabolic enzyme she has just isolated. She obtains kinetic data in the presence and in the absence of two different inhibitors (A and B). The identity of the inhibitors is unknown but we know that one of these is an substrate analog while the other is an alkylating agent. Determine a) Km and Vmax of the enzyme b) which inhibitor is the substrate analog? Which is the alkylating agent? c) Ki for both inhibitors, d) what would be the Vo for this enzymatic reaction at [SI 3x 10M and in the presence of the inhibitor [A] = 2 x 10-, M? Without inhintor With snhiiar With inhibitor H 0 87 067 0 54 045 183 0,49 0.36 026 023 0.48 0.33 0,25 0,20 0.17

Explanation / Answer

c) inhibition constant for A:

kmapp = km + km [I] / ki

ki= km[I]/(kmapp- km)
ki= 4.25x10-4M x 5x10-4M / (1.11x10-3M - 4.25x10-4M)
ki=5.64 x10-4M
inhibition constant for B:
Vmaxapp=Vmax / (1+[I]/ki)
ki=0.889(3.2x10-6) / (2.36-0.889)
ki=1.93x10-6M
d)
v=Vmax x [S]/ [S]+kmapp
kmapp = km + km[I] / ki
kmapp=4.4x10-4 + 4.4x10-4M x2 x10-5M/ (5.64x10-4M)
v =Vmax x[S] / [S]+kmapp
v=0.936 umol/min

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