HeLa human tumor cell cultures were mock-infected (samples 1-3) or infected with
ID: 193706 • Letter: H
Question
HeLa human tumor cell cultures were mock-infected (samples 1-3) or infected with poliovirus for the time periods indicated in the figure (samples 4-10). The cells were labeled with [35Slmethionine for 30 minutes at the end of the incubation periods, and equal amounts of cytoplasmic protein extracts were separated by SDS-polyacrylamide gel electrophoresis. The gel was dried, and autoradiography was performed. (Note: The charges of electrodes during electrophoresis are indicated by and G Figure: 121 Time (h.min) 1 2 3 45 6 7 8 9 10 Samples Questions: 1.- What was the purpose of [35Slmethionine labeling of cells? 2.- What was the initial effect of poliovirus infection on [35 samples 1 and 4)? 3.- What are the bands indicated by the arrows on the right side of the autoradiograph? 4.- What is the effect of longer poliovirus exposure on [35S]methionine incorporation (compare samples 4 and 10)? 5.- What conclusions can be drawn from the results of this experiment? incorporation (compareExplanation / Answer
1. Methionine are used for the tracking of protein production quantitatively and qualitatively before and after the infection of virus.
the labelled methionine provided by idea about the protein quantity and production.
2. is compared to sample 1st and 4th we can clearly visualise that the presence of virus increased the consumption of methionine. Mithionine consumption is increased, this indicates that increase in the production of protein in the presence of virus as compared to in the absence of virus.
in sample first bands are lighter and thin and in sample for bands are dark and thik which reflects the quantitative idea of protein.
3. the bands which are indicated by Arrow reflects the idea of protein of virus because the concentration or thickness of these bands are increased in the presence of viral Load. is the viral load increases the concentration of protein increases it indicates that these are the bands for viral proteins. in other Suns we can say CU proteins are bacterial proteins which are necessary for the production of viral proteins or DNA.
because virus induces or enhances the gene transcription of host because it requires the host machinery for its own replication.
4. Is the virus exposure increases we can see mithionine incorporation increases. it indicates higher viral load consumes the label amino acid for the protein synthesis more rapidly. ( as I mentioned above in upper sentence)
5. the results of these experiments suggest a idea that viral in fact host cells and then use machinery of host for the production of its own copies means reproduction of viruses.
for viral copies formation viruses utilise the components which are available in the medium and then synthesise its own proteins.
that is why is the viral load increases the amino acid consumption is also increased because it utilised by the viruses.
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