13. (Bnus point question): The average molecular weight of DNA is 650 Dalton per

Question

13. (Bnus point question): The average molecular weight of DNA is 650 Dalton per base pair. With the smolecules. 14. (Bonus point question): When we prepare PCR mastermix for amplifying 16S rRNA gene, the final volume 50.0 1 of 1x PCR buffer, for which 10 1 of SX PCR buffer and 27.7 1 of H20 are added (see figure A. below). If now the final volume is 25.011 of 1x PCR buffer, one should add 1 of 5x PCR buffer, and 1 of H20 (see figure B below). PCR Master Mix for 16S rRNA amplification PCR Master Mix 16S rRNA for lificatio ONA 10 DNA 10no/ul Primer 27E (20pmol fL)1.0 Taq Polymerase 10.0 27.7 0.3 250 Taq Polymer A. ToaVoune 0.3 50.0 B.

Explanation / Answer

A. 3.914×1023 molecules

Weight of DNA= 10-6 × 650 × 1000= 0.65gm

Number of molecules in 1 Mole(=1 gm)= 6.023× 1023

Molecules present = 0.65× 6.023×1023 = 3.914 × 1023

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.