10. OK, it is 19th century France and in a back alley of Paris a duel has just t

Question

10. OK, it is 19th century France and in a back alley of Paris a duel has just taken place. The Marquis de Fishey was found dead with a revolver in hand alongside two pools of blood. The "detective primaire" of Paris is on the scene and thanks a new time travel machine; modern forensic techniques have been made available. Three additional characters from the area have been rounded up for questioning and each has given blood. What a foppish group they are! As an expert in forensics, the following evidence that has been presented to you: Blood samples found Victim's Blood The USUSAL SUSPECTS" Duke de Comte Marquis Marquis de Fishay At the scene A) B) Who shot Marques de Fishey? In detail (please be to the point here) describe the technique that was used in determining the culprit. Include how the bands in the above gel were obtained and what type of "junk" DNA was used in this technique

Explanation / Answer

Answer

1. Comte temptuous

2. DNA fingerprinting

1. Extracting DNA from Cells
2. PCR Amplification
3. Treatment with specific restriction enzymes
4. Run the fragments in the Gel electrophoresis.
5. Transfer on to southern blot
6. Add radioactive probe.
7. Analysis in X-ray film

VNTR - variable number tandem repeats used in the DNA finger prints.

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