Proteins can be separated and purified by one of three criteria: 1) Size -larges

Question

Proteins can be separated and purified by one of three criteria: 1) Size -largest elute first 2) Charge-either anion (cations fixed on the immobile phase) or cation (anions fixed on the immobile phase) exchange (least attractive elute first) 3) Affinity- highly specific binding (unbound proteins elute) If this column was based on charge (ion exchange with cations fixed on the immobile phase), which protein has the highest positive charge (solid or hash)? The charge on the peptides can be adjusted by changing the pH of the mobile phase. TT T T T With size-exclusion, small molecules mover over and THROUGH the resin (immobile phase), keeping them inside the column longer.

Explanation / Answer

Since the column is filled with cations, which will attract anions (negatively charged) and hence will elute later, while the cations or positively charged will be repelled and eluted first. So solid lines represent more positive charged.

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