1) Consider two enzymes Bert (solid line) and Ernie (dotted line) that exhibit t
ID: 194206 • Letter: 1
Question
1) Consider two enzymes Bert (solid line) and Ernie (dotted line) that exhibit the kinetic behavior shown in the following graph:
b) Suppose you doubled the concentration of Bert from that used to generate the curve shown in the figure. What values (give numbers and units) of Km and Vmax do you expect to measure?
b) Late one evening in the lab, you run two reactions labeled X and Y, adding Bert to one and Ernie to the other. After recording the data in your lab book, you realize that you forgot indicate which enzyme was added to each reaction.
Given what you know about the enzymes Bert and Ernie, can you determine which enzyme was used in Reaction X and Y with the information shown above? If not, explain what more information is required.
0.8 -Bert ---Ernie 2 0.6 2 0.4 0.2 0 10 20 30 40 9 0 Substrate Concentration (uM)Explanation / Answer
Vmax is defined as the maximum initial velocity or rate of reaction at which enzyme catalyzes the reaction whereas Km is defined as the amount of substrate at which initial rate of reaction is half of its Vmax.
a)so from graph,
expected Vmax value of Earnie =0.6 uM/min and Km = 15uM
expected Vmax value of Bert =0.4 uM/min and Km = 4uM
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