What methods were used to identify replication origin binding proteins in the Be

Question

What methods were used to identify replication origin binding proteins in the Bell and Stillman paper?

affinity chromatography, glycerol gradient sedimentation and DNA footprinting

affinity chromatography,SDS-PAGE, DNA sequencing

glycerol gradient sedimentation, DNA footprinting, recombinant DNA expression

glycerol gradient sedimentation, SDS PAGE, protein sequencing

affinity chromatography, glycerol gradient sedimentation and DNA footprinting

affinity chromatography,SDS-PAGE, DNA sequencing

glycerol gradient sedimentation, DNA footprinting, recombinant DNA expression

glycerol gradient sedimentation, SDS PAGE, protein sequencing

Explanation / Answer

DNA fingerprinting used to determine the sequence of DNA binding protein sites. Bell and stillman identified the long sought initiator protein that binds to replicator sequences to initiate DNA replication in eukaryote. Affinity chromatography is also used in the replication origin binding proteins in the Bell and Stillman paper.

Hence option (a) is correct.

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