We want to cut the following DNA sequence into 3 fragments. Which restriction en

Question

We want to cut the following DNA sequence into 3 fragments. Which restriction enzyme should we use? (Use the digestion pattern table from the previous question.) 5'-GCGGGCCCCATTTATACCCTCCGCGCGCCGGACCGGGGTCCGCAAGGCGCGGCGGTGCACAAGCAGTTGACGACTGACCGCCGTGTATTCGTTATGGCACCAGGGAGTTCGGGCCGAGTCAATGGAGCTCGCAATACGGAGTTTACCGCGTCCTGCCCCAACCGACGGGCTGTGATCGACCACGAGCCAAGCCATTGCCTCTCGGGCACGCCGCTACAGCGATTACGAAAACCTTGCGGGGCATGGCTGCGGCCCGTTCAGCCACGTCCGAGGGCAGAAACCTGCCCCCATCCGTATGCTCGGCTATCTTCTACCCATCCCCGGGGGCTAGGCAGGTCGTGGGGTGCGGAGGAGGCTCTCGATCATCCCGTGGGACATCAACCTTCCCCTTGATAAAGCGCCCCGCTCGGGTGTGGCAGAGAGGACGCCTTCTGAGTTGTGCCATCCCTCGACCTCACCAAAGCTTGCTACCAATAATTAGGATCCCTGCCTCGCGACA-3'

AluI

EcoRI

BamHI

None of these restriction enzymes will cut this DNA sequence into 3 fragments.

SmaI

All 6 restriction enzymes will cut this DNA sequence into 3 fragments.

HindIII

HbaI

AluI

EcoRI

BamHI

None of these restriction enzymes will cut this DNA sequence into 3 fragments.

SmaI

All 6 restriction enzymes will cut this DNA sequence into 3 fragments.

HindIII

HbaI

Explanation / Answer

None of these restriction enzymes will cut this DNA sequence into 3 fragments.
Noncutters: EcoRI, HbaI
SmaI recognizes CCCGGG makes 1 blunt cut at position 322
BamHI recognizes GGATCC makes 1 5' overhang cut at position 481
HindIII recognizes AAGCTT makes 1 5' overhang cut at position 461
AluI recognizes AGCT makes 2 blunt cuts at positions 127, 463

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