The primers we will use for PCR will... (choose the most correct answer) Will ha

Question

The primers we will use for PCR will... (choose the most correct answer) Will have restriction sites that exist on the vector Because of this, the primers will not be annealing fully and completely to the genomic DNA Will both have the same restriction site incorporated. That will ensure proper insertion into the vector, which would be also cut with the same enzyme. 0 Incorporate restriction sites that are naturally occuring in the yeast genome Ocompletely and fully anneal to a complementary sequence in the yeast genome. If there is no full overlap and annealing, the PCR will not be successful Will have some random sequence that does not anneal to anything, right in the middle of the gene of interest. This is to make sure that the restriction enzvme can grab onto something before it can cut the DNA

Explanation / Answer

Answer: completely and fully anneal to a complementary sequence in the yeast genome. If there is no full overlap and annealing, the PCR will not be successful.

ncorporation of restriction sites to primer is a matter of choice in cloning but the main features of a primer is it anneals to the complementary site so that polymerase can recognise and elongate the DNA.

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