7. Cakculate the final concentration it 2.00 L of 3.00 M Naci. 4.00 L of 1.50 M

Question

7. Cakculate the final concentration it 2.00 L of 3.00 M Naci. 4.00 L of 1.50 M NaCI and 4.00 L of water are mixed. Assume there is no volume contraction up 18. . How do you prepare a 50-ml solution of 20% (wi) Sodum Dodecyl Sutate (SDS)? 19. How much ampiciin do you need to make 100 ml of 100mgiml ampicillin? Ampicilin dissolve in water 20. You have a stock solution of NaCI at 4M and you want a 500mL of a working solution of 20mM. How much stock solution do you need to add to how much mixing water to obtain the final working solution? 21. You want 1mM Tris-Hd and 25u/M NaCI in 100ml and you have 1M Tris-HCI and sold NaCi(MW- 58.44 g/mol). What do you do? 22. How many grams of calcium chloride (CaCla) would you need to make 250 mL of a 0.25 M solution? Molecular weight of Cacl,-110.98 g/mo 23. Calculate the molarity of a solution prepared by mixing 250 mL of 0.75 M H2S04 with 150 mL of water 24. You have 50X solution and you want to make 500ml of 1X solution for your experiment? How much of the 50X solution do you use? How much water do y need?

Explanation / Answer

17. 2L of 3M NaCl
1M = 58.44g in 1000 ml
3M = 175.32 in 1000 ml
3M,m 2L solution = 350.6 g NaCl.

4L of 1.5M NaCl.
1.5 M = 87.66g in 1l
4L of 1.5M = 350.6 g NaCl.

Total NaCl = 350 + 350 = 700 g = 11.97 M
Total water = 2+4+4 = 10 L
So concentration = 700 g / 10 L = 70g /L = 1.2M

18. 20% SDS of 50ml solution
so we need to weight SDS to make it 20% in 50 ml.
20g in 100ml = 20%
10g in 50ml = 20%

19. 100ml of 100mg/ml ampicillin.
weight 10g (10000mg) of ampicillin and dissolve in 100ml of water; it becomes 10000mg/100ml = 100mg/ml.

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