Brightfield Microscopy (continued B. Matching Questions Match the lens (condense

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Brightfield Microscopy (continued B. Matching Questions Match the lens (condenser high dry, low power, oculor ar of immersion) to its ANSWERS description. Cholces may be used more tho once Matching 1. This objective lens provides the highest magnification 2. This objective lens provides the second-highest magnification 3. This objective lens provides the lowest magnification 4. This objective lens has the shortest working distance 5. The coarse focus knob should be adjusted only when using this objective lens 6. This lens collects and focuses light from the lamp onto the specimen 4 on the slide 7. This lens, also known as the eyepiece, often comes in pairs 9. A diaphragm is used to regulate light passing through this lens. 1. Acetone is the safest solvent for cleaning an objective lens 5. 8. Diopter adjustments can be made to this lens. C. True-False 2. Only lint-free, optically safe tissue should be used to wipe off mi - 8. 3·The total magnification capability of a light microscope is only 4. The coarse focus knob can be used to adjust the focus when using True-False 5. Once focus is achieved at one magnification, a higher-power objec- 1 croscope lenses limited by the magnifying power of the lens system. any of the objective lenses tive lens can be rotated into position without fear of striking the slide 3. D. Multiple Choice Select the onswer thot best compictes the foWowing statements 4. 1. The resolving power of a microscope is a function of a. the magnifying power of the lenses. b. the numerical aperture of the lenses. c. the wavelength of light. d. Both (a) and (b) are correct. e. Both (b) and (c) are correct. 5. Multiple Choice 2. The coarse and fine focus knobs adjust the distance between a. the objective and ocular lenses b. the ocular lenses. c. the ocular lenses and your eyes. d. the stage and the condenser lens. e. the stage and the objective lens. 3. 3. A microscope that maintains focus when the objective magnifica- tion is increased is called a. binocular b. myopic e. parfocal. d. refractive. e. resolute. 10

Explanation / Answer

B. Matching questions

Ans:

1. This objective lens provides the highest magnification: Oil immersion.

2. This objective lens provides the second highest magnification: High power.

3. This objective lens provides the lowest magnification: Low power.

4. This objective lens has the shortest working distance: Oil immersion.

5. The coarse focus knob should be adjusted only when using this objective lens: Low power.

6. This lens collects and focuses light from the lamp onto the specimen on the slide: Condenser.

7. This lens, also known as eyepiece, often comes in pairs: Ocular.

8. Diopter adjustment can be made to this lens: Ocular.

9. A diaphragm is used to regulate light passing through this lens: Condenser.

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