3. pelleted using the procedure shown below. Succinate Dehydrogenase (SDH) was u

Question

3. pelleted using the procedure shown below. Succinate Dehydrogenase (SDH) was used as the organelle marker. Fraction SSDH Fraction Total SDH/fraction ASDHProtein RSA (g/min) 2,500 pellet 2505 70,000 pellet 150 300,000 pellet 375 2 cytosolic (U/mL) vol (mL) 30 20 15 35 15 35 20 (a) Complete the missing information and calculate the Relative Specific Activity (RSA) of the fractions (b) Plot the data as a bar histogram of RSA (y-axis) versus % protein (x-axis). (c) What fraction is enriched in mitochondria?

Explanation / Answer

A)

RSA=  

enzyme concentration [u/ml]

______________________ = specific activity [u/mg]

protein concentration [mg/ml]

b/

c) 300,000 pellet is enriched in mitochondria since it has highest enzyme activity

Fraction SDH (U/mL) Fraction vol (mL) Total SDH/Fraction (U) Delta SDH (U) Protein % RSA 2500 pellet 250 5 50 30 8.3333333 70000 pellet 150 15 10 20 7.5 300000 pellet 375 2 187.5 15 25 cytosolic 35 20 1.75 35 1

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