Pre-lab Enzyme 8. Wh at enzyme will you be studying in lab today? Where can you

Question

Pre-lab Enzyme 8. Wh at enzyme will you be studying in lab today? Where can you find this enzyme? 9. Write the reaction catalyzed by this enzyme. Do you expect this enzyme to cellulose (polymer of glucose)? Explain. hydrolyze 10. When an enzymatic reaction is in progress, do you expect to see an increase, decrease or no change in the concentration of each of the following: Substrate- Product- Enzyme- 11. Maltose is produced by (condensation, dehydration, hydrolysis..) of starch. Carefully read the part I of the lab: Assay of Hydrolysis of Starch by Amylase. should be high, low or constant? 12. If the enzyme activity of amylase is high, then is the concentration of maltose produced 13. What is the purpose of Tube B? You will use Tube B when you use what instrument to read your results at the end of the experiment? 14. What 3 different reagents or substances will be put in Tube 25 C for the enzyme reaction? What 2 different reagents or substances will be put in Tube B for the enzyme reaction? 15. To estimate the amount of maltose produced by the enzymatic reaction, you will add 1000 L of what chemical reagent?

Explanation / Answer

8.
Enzyme = Alpha amylase
Source = Saliva

9.
Amylase catalyzes the conversion of starch to glucose dimers (Maltose).
Starch -----> Maltose

10.
During an enzymatic reaction,
Substrate concentration decreases.
Product concentration increase.
Enzyme concentration does not change.

11.
Maltose is produced by the hydrolysis of starch.

12.
If the enzyme activity of amylase is high, the concentration of the product i.e, maltose will also be high.

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