In a replica plating, a master plate with bacteria was plated onto two plates th

Question

In a replica plating, a master plate with bacteria was plated onto two plates that had the antibiotic ampicillin. The plates stamped both had a lot less bacteria than the master plate. What does this say about the bacteria following Darwin or Lamarck evolution? In a replica plating, a master plate with bacteria was plated onto two plates that had the antibiotic ampicillin. The plates stamped both had a lot less bacteria than the master plate. What does this say about the bacteria following Darwin or Lamarck evolution?

Explanation / Answer

In the master plate, there is no antibiotic. So, no selection pressure.
Hence all bacteria can grow.

In the passaged plates, ampicillin is present. It serves as a selection pressure.
So, only cells that can degrade ampicillin can survive.

Darwinian evolution:
Bacteria that evolved AMP resistance by mutations are selected by the selection pressure (Ampicillin).

Lamarckian evolution:
Some bacteria in the original population was preexposed to ampicillin and they could metabolize it.
Only these colonies grew on the selection plate.

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