18. Cytochrome C has a molecular weight of 20,370 and hemoglobin 68,000, which p

Question

18. Cytochrome C has a molecular weight of 20,370 and hemoglobin 68,000, which protein will fractionated from a saphadex GA-25 column (exclusion limit of 5000) a. Cytochrome C b. Hemoglobin c. a, b d. None of them 19. In all type of chromatography, the stationary phase may be b. Gas c. solid d. a and b e. a and c a. Liquid 20. The mass of bead packed within the column chromatography is a. Void volume b. Elution volume c. Exclusion limit d. Column bed 21. Following proteins are eluted from a diethylaminoethyl(DEAE) cellulose resin (an anion exchanger), how many distinguishable fractions you would get at the end of fractionatior The pi (isoelectric point) is listed for each compound. 1-Cytochrome c pl: 10.6 2-Hemoglobin pi: 6.8 3-Serum albumin pi: 4.9 Myoglobin: pi: 5.9 5-pepsin, pi: 1.0 6- erepsin, pl: 1.0 a. 2 b.3 c. 4 d. 5 e. 6 22. In a gel permeation column(size exclusion), fractionation processes are based on a. Size of molecule b. Charge of molecule c. Charge of the resin d. Ligand-molecule e. All of the above 23. In a Sephadex G-100 column determine the order of eluted following proteins (first t Exclusion limit: 100,000 d)

Explanation / Answer

18.
Option D is correct.
Exclusion limit of G-25 column = 5000 Da
Cyt C = 20370 Da
Hb = 68000 Da
So, both these proteins can not pass through the interstitial spaces of the column.

19.
Option E is correct.
A stationary phase can be liquid or solid.

20.
Option D is correct.
Column bed = Mass of bead packed within the column

21.
Option D is correct.
Five fractions with five specific pI.
Pepsin and Erepsin come in the same fraction.

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