Assume you stain clostridium by applying a basic stain, carbolfuchsin, with heat

Question

Assume you stain clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endoscopes are _1_ , and the cells are stained _2_.

A) 1- red; 2- black
B) 1-black ; 2- colorless
C) 1- colorless; 2- black
D) 1- red; 2- colorless
E) 1- black; 2- red

I am confused that the answer is D, because the textbook says that Endospores cannot be stained by simple staining ot gram staining because the dyes dont penetrate the enospores wall..

Explanation / Answer

you are right by simple staining endospores cannot be stained. for staining the endoores high heat or pressure has to be applied.Steam and heat are needed to allw the stain to enter the endospore. Water and decoloriser cannot was the stain away

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