and auestions 14-17, use the translation cartoon below showing an eukaryotic mRN
ID: 195432 • Letter: A
Question
and auestions 14-17, use the translation cartoon below showing an eukaryotic mRNA transcript, transpeptidation as well NH RsCH NH RcCH NH NH3 c-G-G-A-c-6-c-u-G-C-A-C-G-A-G-G-u- 14. (4 pts) Show the mechanism for the next transpeptidation reaction on the figure above, by drawing arrows. 15. (3 pts) Write the name of the ribosomal subunit that is involved in accelerating the reaction described in question 14. 16. (4 pts) Identify the amino acid that has a R group labeled Rs, by writing the 3-letter code. 17. (4 pts) After translocation, what codon will be in the P site? (Write the sequence). 18. (4pts) In translation the growing peptide is attached to a tRNA molecule, what (specifically) hydrolyzes the completed peptide from tRNA? Write your answer in the space providedExplanation / Answer
15. The large ribosomal subunit is involved in peptide bond formation during translation.
16. Rs = Glycine = Gly = G
17. After translocation, A site becomes empty and newly added amino acid enters the P site.
The codon after translocation = GCA
18. Peptide release involves the release of completed protein from the peptidyl tRNA at the end of translation. It is catalyzed in the large subunit of the ribosome. This process also requires class I release factor.
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