Select all of the correct answers regarding differential stains. Select one or m

Question

Select all of the correct answers regarding differential stains. Select one or more: O a. Differential stains are much easier to use than so-called "simple" stains, which require precise timing. O b. Differential stains allow a microbiologist to separate microbes into groups c. Differential stains, especially Gram's stain, does not provide much information if the counter-stain safranin is not used. d. Differential stains facilitate the visualization of some of the structural components of bacteria. O Next page Previous page

Explanation / Answer

1. b) Differential staining such as gram staining helps microorganisms to be seperated into gram negative and gram positive bacteria. Gram stain uses two dyes - crystal violet and safranine.

c) Differential staining such as gram staining is ineffective if safranin is not used. Safranin functions as counter stain in gram staining. Gram negative bacteria is counter stained using safranin. Safranin is also used in endospore staining.

d) Differential staining helps in identifing the structural components of bacteria such as cell wall. Gram positive bacteria consist of thick cell wall and stains with crystal violet and gram negative bacteria have thin cell wall and does not retain purple stain. These gram negative bacteria is counter stained using safranin.

2. False, simple stain provides basic morphology of bacteria. Mostly, simple stain helps to understand the bacterial shape. Example - methylene blue.

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