the method of Bradford, is the second assay we will be performing in this lab an
ID: 195740 • Letter: T
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the method of Bradford, is the second assay we will be performing in this lab and is highly sensitive and blue) when binding to protein occurs. The Bio-Rad Protein Microassay, based ry rapid Biochemistry Chemistry 354 Laboratory Exercise 3- Protein Assays Purpose To determine the protein concentrations in solutions by the Lowry and Bradford assays Materials Needed Spectrophotometer, cuvette tips, waste beaker, distilled water, Bovine Serum NaH PO4 pH 7.0), Phosphate buffer for dilution of the BS Reagent B (diluted Folin-Ciocalteau Reagent or Folin's Reagent). Bio-Rad Dve Rea solution of unknown concentrations in 10 mM NaH POs pH 7.0 Method I. Lowry Assay s, vortex mixer, marker pen and tape, micropipettes (P-10, P-20, P-200, P-1000) and Albumin (BSA) standard solution (2.00 mg/ml in 10 mM A (10 mM NaH POa pH 7.0). Lowry Reagent A, Lowry gent solution and BSA Name: Experiment title I. Please answer the following pre-lab questions before you come to each lab. The answers have to be turned in at the beginning of the each lab class. Please print and attach this question/rubric page to each and every pre-lab report. Describe the goals of the experiment. If the experiment has multiple parts. Explain clearly the a. goal of each part of the lab b. There are key theoretical principles which have been used to design the lab procedure you will be doing. These theoretical principles are important in achieving the goals of lab mentioned in part a. Explain each key theoretical principle involved in this lab and how it is useful to achieve the goals of the experiment? c. Explain the key equations used in this lab and the terms involved in the equation d. List all key instrumentation used in the lab and explain how it was used in the lab exercise Goals -Max (3 points) Principle-Max 6 (points) Equations - Max (3 points) Instrumentation - Max (3 points) CHEM 354 Lab Report Rubric for CLS questions: Does Not Meet Meets expectations Exceeds Expectations Score Goal/background is Goal/background is Goal/background described Expectations informatiorn demonstrates nderstanding of the Goal described he ndsufficientlyExplanation / Answer
1. Series of dilution of BSA is prepared.
2. BSA standards are made by adding BSA with Lowry reagents
Protein samples are added with BSA
O.D. taken at 750 nm
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