1. The LIVE/DEAD viability stain has been used to study Pseudomonas aeruginosa b

Question

1. The LIVE/DEAD viability stain has been used to study Pseudomonas aeruginosa biofilms, as discussed in the text. How could the LIVE/DEAD stain described in the chapter be helpful in specifically examining the regulation of explosive cell death in P. aeruginosa biofilm formation?

A.You could stain biofilms at different stages of development with and without exposure to antibiotics to examine patterns of cell death because the living cells would stain green.

B.You could expose biofilms to antibiotics and then stain them to see which parts were harmed the most.

C.You could stain biofilms throughout the stages of development to determine normal patterns of cell death throughout the stages.

D.You could stain many biofilms, all at a similar stage of development.

2.

Based on your understanding of the chapter material and the information in the passage, what are some of the different ways that researchers could study the gene expression underlying explosive cell death?

Select all correct answers.

View Available Hint(s)

Select all correct answers.

-Tagging of genes for virulence factors -Removing the prophage from P. aeruginosa cells -Tagging of genes for lysis enzymes -Use of reporter genes

Explanation / Answer

Genes encoding for lysis enzymes may be tagged and their expression pattern may be reported with the help of some reporter gene. Reporter genes will indicate the presence of lysis factors or proteins. Hence option C and D are correct

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