1.)Your professor has drawn the picture below as a diagram of a eukaryotic gene
ID: 196241 • Letter: 1
Question
1.)Your professor has drawn the picture below as a diagram of a eukaryotic gene that is being transcribed and translated.
Ignoring what is wrong with this diagram, which letter most accurately shows the location of the 5' CAP on the RNA?
2.) Your professor has drawn the picture below as a diagram of a eukaryotic gene that is being transcribed and translated.
Ignoring what is wrong with this diagram, which letter most accurately shows the location of the poly A signal in the DNA?
3.) Your professor has drawn the picture below as a diagram of a eukaryotic gene that is being transcribed and translated.
Ignoring what is wrong with this diagram, which letter most accurately shows the location of the RBS in the DNA?
4.) Your professor has drawn the picture below as a diagram of a eukaryotic gene that is being transcribed and translated.
What is wrong with this diagram?
A length of DNA to be transcribed- 100 nucleotidesExplanation / Answer
1. The mRNA is transcribed in 5’ to 3’ direction from the template strand (3’ to 5’). The 5’ CAP is a specially altered nucleotide attached to the 5’ end of the mRNA. The 5’ end has a free phosphate as it the first nucleotide incorporated in the chain. An altered GTP is added to the 5’ end by the enzyme guanyl transferase. The 5’ end of mRNA is denoted by G.
Right option is G.
2. The poly-A tail is a long chain of adenine nucleotides added to mRNA molecule after transcription. It increases the stability of RNA. It is added to the 3’ end of the mRNA. The 3' end of the transcript is cleaved to free a 3' hydroxyl. To this 3’ hydroxyl end, poly-A polymerase adds a chain of adenine nucleotides. The 3’ end of the mRNA in the figure is denoted by H.
Right option is H.
3. The ribosome-binding site (RBS) is present on the mRNA. RBS is a set of nucleotides that are present upstream to the translation start site, where RNA polymerase binds. RBS is required for assembly of ribosomes. RBS is not present in DNA.
Right option is none.
4. mRNA in eukaryotes is synthesized in nucleus as heterogenous nuclear RNA (hnRNA). A poly A tail and a 5’ cap is added. Splicing of introns occurs either during transcription or after transcription. The mature mRNA is transported out of the nucleus to the cytoplasm. The diagram shows simultaneous transcription and translation occurring in the nucleus. This occurs only in prokaryotes which lacks a nuclear envelope.
Right option is: the mRNA should not have ribosomes on it since, in eukaryotes, translation occurs in the cytoplasm only after completed mRNAs are transported out of the nucleus.
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