hjsp?course-assessment · c || M course Invitation: Remaining Time: 59 minutes, 3
ID: 196373 • Letter: H
Question
hjsp?course-assessment · c || M course Invitation: Remaining Time: 59 minutes, 34 seconds. Question Completion Status The commercial product Frostban contains a genetically engineered strain of O Escherichia col. O Saccharomyces cerevisige 0 Thermus aquaticus O Pseudomonas syringae O Pseudomonas fluorescens QUESTION 2 When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA? PCR O Cloning O The Sanger Method O Southern blot hybridization O Microarray analysis QUESTION 3 Analysis of DNA fragments in gel electrophoresis is based on O larger fragments move slowly and remain closer to the wells. O DNA has an overall negative charge and moves to the positive pole O DNA fragments are stained to see them. O an electric current through the gel causes DNA fragments to migrate. O All of the choices are correct. QUESTION 4 Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects O Escherichia co Saccharomyces cerevisiae O Thermus aquaticus Pseudomonas syringae O Pseudomonas fluorescens QUESTION5 Which step involves transformation? o target DNA removed from cells and isolated O cloning host takes up a plasmid target DNA and plasmid O desired protein is produced by cloning host O gene is ampified by multiplication of cloning host treated with the same restriction endonuclease QUESTIONExplanation / Answer
1. Pseudomonas syringae is a bacteria varient of which may used.
2.Sanger method is a technique to identify sequences of DNA. Option C is right.
3. All the points are true. DNA is negative and stained with EtBr.
4. Recombinant of E.coli may secreate insecticides e.g. E.coli JM103.
5.for transformation both the target And host DNA are digested ewit same restriction enzyme.
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