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(1Upts) Vesicles in Set C contain a novel transport protein that uses a Ca2*-ele

ID: 196463 • Letter: #

Question

(1Upts) Vesicles in Set C contain a novel transport protein that uses a Ca2*-electrochemical gradient to drive glucose transport against its concentration gradient. You wish to answer the question: 1s the transport protein a symporter or an antiporter?" You have a method to monitor glucose movement into vesicles, and you can set the initial concentrations of glucose and Ca2+-ions inside and outside the vesicles. Using these tools design an experimental plan to answer the underlined question. Be sure to include: - Initial conditions with relative concentrations of Ca2 and glucose inside and outsid e of the vesicles How you would interpret results from your experiments to answer the underlined question

Explanation / Answer

Answer:

To address the question whether the transporter protein is a symporter or an antiporter, we can design below experiment:

Monitor the influx of radiolabeled glucose into liposomes (glucose transport into liposomes).