Read the clinical case below and anower the quesions e Cheryl Tay lor, the atien

Question

Read the clinical case below and anower the quesions e Cheryl Tay lor, the atiending physician at the emagmcy oom looked up fhom a chart o really oul of whack While Casey.jest one weck ago he was perfecly hely Se1puel and wemied Cascy was seeping in her mothar'sarms in the tin ahicle Abricf glance and ber skin did have the dry, wrinkly feel of seve dehobem Caey's mader said hr would she gst the Neod The levcs he Hood n cause her heat to beat iegslarly ar even sap ahogethe arg cellular waste products that s" omuly removed by aiheys. Chey's BUN was 52 mgdL; the normal range i, 5-15. H acatinite was 3., Nenal 0.146. Thee valios, plus I askod the surse te check her blood pressurew 13582-suly elevatod for a 2- building her blood supply. Bat Cascy's nod blood ell c was even lewer than enxpectad And od slood cell who got sick and The iness pread through we don't know how which did so

Explanation / Answer

1. E.coli O157:H7 is the causative agent for the patient illness (Hemolytic uremic syndrome- HUS).

Some strains of E.coli will produce a toxin called Shija toxin. Shija toxin secreting E.coli cells will trigger HUS infection.

Other serotypes such as Shigella, Salmonella and campylobacter will cause food borne illness (gastrointestinal illeness ). It may also trigger HUS.

Choices:

1.E.coli

2.Shigella

3.Campylobacter

4.Salmonella  

Correct option is 1.E.coli

2.

positive

pink to red colonies

S.no Biochemical test Principle Inference E.coli E.coli o157:H7 1 SMAC( Sorbitol Mac Conkey Agar )Test Fermentation of sorbitol leads to pink to red colonies

positive

pink to red colonies

positive negative 2 MUG Assay Enzyme beta glucuronidase will cleave the substrate MUG(4-methylumbelliferyl-B-D-glucuronide) leads to release of fluorescent product. presence of fluorescent product positive negative 3 O157 latex test O157 antigen reacts with anti sera and produce agglutination presence of agglutination no agglutination agglutination

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