One of the available treatment conditions was a T. vorax growth medium adjusted

Question

One of the available treatment conditions was a T. vorax growth medium adjusted to pH 5. For what purpose was this solution provided?

Update: This is not a disease. It is a lab treatment, as in, not the control. The question is asking the significance of pH 5 relevative to the cilliate organism T. vorax.

A. To simulate saltwater encroachment of a freshwater environment.

B. To prevent digestion of the India ink particles in the phagolysosome.

C. To simulate acidification of a freshwater environment due to acid rain.

D. To serve as the negative control since food vacuoles are not formed under acidic conditons.

A. To simulate saltwater encroachment of a freshwater environment.

B. To prevent digestion of the India ink particles in the phagolysosome.

C. To simulate acidification of a freshwater environment due to acid rain.

D. To serve as the negative control since food vacuoles are not formed under acidic conditons.

Explanation / Answer

The acidity and basicity of a solution are determined by measuring the pH. If the pH is less than 7 then the solution is said to be acidic and if the pH is above 7 then it is an alkaline solution.’

A laboratory treatment protocol has been followed using T. vorax growth medium wherein the pH was adjusted to 5. This makes the medium more acidic. Now the question, what is the role of pH 5 in the ciliate organism T. vorax. T. vorax is a suitable organism to observe phagocytosis in a compound microscope. When this ciliate becomes hungry, it engulfs several particles to their oral groove and forms food vacuole through phagocytosis.

Hence, final answer is to serve as the negative control since food vacuoles are not formed under acidic conditions. Food vacuoles are formed more at PH more than 3.5

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