uled for 3/5). Please answer all the questions to the r abilities and remember t
ID: 196763 • Letter: U
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uled for 3/5). Please answer all the questions to the r abilities and remember that this quiz is open extbook. You will only be able to take this quiz once ould use it as an opportunity to compare your answe he answer key and gage any gaps in your knowledge. are any issues, please come talk to me during office h Question 1 0 pts When researchers want to isolate (remove but keep intact and functional) an integral membrane protein from a cell membrane, they often use an amphipathic detergent such as Triton X-100 (shown below). Why do you think that such a detergent would be useful for keeping integral membrane proteins "happy" during the extraction? CH3CH H3C-C-CH-C CH3 CH3 (Nonpolar Region) Polar Region)Explanation / Answer
During the extraction of integral membrane proteins, it is essential that the protein remains in its native confirmation. Detergents such as triton-x 100 are amphiphathic in nature which means it has a hydrophilic head and a hydrophobic tail as shown in the structure given a nonpolar hydrophobic group and a polar hydrophilic group. When the detergent will be added to the membrane, the hydrophilic group will provide water solubility wheras the hydrophobic regeion will help in partitioning of the nonpolar lipid bilayer. Hydrophobic group will also mask hydrophobic portion of proteins to retain their native confirmation and avoid formation of aggregates.
Once the dtergents are mixed with the membrane, lipids of the bilayer mixes with the detergent and forms micelles which resemble the lipid bilayer and the proteins of the membrane forms complex with the detrgent micelles and gets the same environment as in the lipid bilayer. This allows the proteins to remain stable and in its native confirmation.
PS. Though solubiliztion of protein requires immense efforts. It is very important to select appropriate detergent for the solubilization of specific protein.
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