O e The figure below reprosents a Westen Blot used to diagnose a patient with an
ID: 196984 • Letter: O
Question
O e The figure below reprosents a Westen Blot used to diagnose a patient with an HIV infection The antbody recognizes several proteins that ar present on the outer coat, or capsid, of the HIV virus On the Western Blot below (let), lanes 1 and 2 represent a known HIV positive and HIV is HIV positive, negative, or Indeterminate is listed in the table below What is the stalus of patients, A, B, and C from the data in the Western Blot 2 individual patients. The criteria for establishing whether a patient Ep160 OR gp120 positivity present, gp 160 gp 120 p65 9p41 p31 p24 O A-positive, B-negative, C-intermediate Anegativo. B-intermediate, C-posiivo O A-negative, B-positive C-positive O A intermediate. B-posilive, C-negativeExplanation / Answer
Patient A is negative because there are no bands in the lane A
Patient B is intermediate because there are bands in the lane B ,band is present at p24 but absent at p160 or p120 .so the pattern of bands doesnot meet the criteria , so B is intermediate.
Patient C is positive because bands are at p160 , p120 and p24 .so C is positive for HIV.
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