Question 3 (10 points) pRL27 is a suicide plasmid and has a conditional origin o

Question

Question 3 (10 points) pRL27 is a suicide plasmid and has a conditional origin of replication called oniR6K. It only replicates in Pir+ strains of E. coll. If you add the translational inhibitor antibiotic streptor yan to a culture of Pir+ E, eoli cells that contain pRL27, will te copy number of pRL27 increase in tht ebence of cell desion, Ephn-hy or ihr not in the context of the mechanism of replication for this on Save Question 4 (10 points) Mycoplasmas lack peptidoglycan, You isolate a new strain of Mycoplasma and find that it is inherently resistant to penicilin. Do you think this strain will also be resistant to vancomycin? Explain why or why not in the context of mechanisms of action of penicilin and vancomycin.

Explanation / Answer

3.
The plasmid replication requires proteins.
If protein synthesis is inhibited by streptomycin, the plasmid can not undergo replication.

4.
Penicillin kills microbes by inhibiting their cell synthesis (Peptidoglycan synthesis).
Vancomycin inhibits the second step of peptidoglycan synthesis.
Mycoplasma are resistant to both Penicillin and vancomycin as they do not possess the cell wall.

5.
If a gel mobility shift assay is performed with EcoRI enzyme and a DNA fragment that contains multiple EcoRI recognition sites, we would expect a smear in the gel. This is because the enzyme binds to DNA and cleaves it into pieces.

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