10:40 PMM cluster80-files.instructure.com Verizon LTE 59% 20 20 90 Quantificatio

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10:40 PMM cluster80-files.instructure.com Verizon LTE 59% 20 20 90 Quantification of haploid anaphase cells of the indicated genotype with the new SPB in the bud in the young SPB lineage Gretta old SPB lineage (red) and average of both SPB lineages(blu%) (n·3 independent experiments with a total of-t20 cells per genotype analysed). All statistical significances were calculated using one-way ANOVA, P0.0001"P 0001,'? 005; NS, not significant. All error bars represent mean·sd Source data are avalable in Supplementary Table 1. Supplementary Figure 3 : NuA4 promotes SPB inheritance likely by modifying Nudl and 25 (Quantification of anaphase cells of indicated genotype segregating the new SPB into the bud (h6) (n-3 independent experiments, except wT, SPC72-TAP. "elia-44, ya 9 nud}.44, NUDI-TAP and NUDIK3SR yaf9A. 4, with a total of >120 cells per genotype analysed). One way ANOVA was performed to test significance, ""P

Explanation / Answer

the supplementary figure 3 describes Inheritance of the SPB gene and that this inheritance is increased by the promoter NuA4. The mechanism pathways that are modified in increasing this inheritance are the Snc72 pathway as well as the Nud1 pathway. In the graph below, there are different cells used, with distinct genotypes, which include, WT, spc 72-2, SPC72, SPC72KTOPR, yaf9delta, nud1-44, nud1-44 yaf9delta, NUD1, NUD1K35R, NUD1KETR, NUD1K35R yaf9delta. These genotypes are used such that the effect of these two mechanisms, could be understood in these specific cell types. This has been checked in the anaphase phase of cell division, where the percentage segregation of the SOB genotype has been attempted to be understood.

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