I need help with 6.2D question 1- 5. please help 5, Is the nucleus visible? If s

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I need help with 6.2D
question 1- 5. please help

5, Is the nucleus visible? If so, where in the cell is it found? nucleus might be found in its current location Suggest one reason why the 6.2D Onion Epidermis In this procedure, you will observe plant cells comprising the inner epidermis of an onion Alu cepa) storage leaf. Because these cells are not normally exposed to light, they do not carry out photosynthesis although they have the genetic information (genes) to do so. Epidermis s the outer most layer of tissue surrounding the soft structures of plants: bodies, leaves, roots, and many stems of plants. Onion leaves have two layers of epidermis: the outer and the inner layers. This procedure will showcase the nucleus of each epidermal cell. 1. Obtain an onion, several clean glass slides, several cover slips, a pair of forceps, a bottle of dH O, a transfer pipette, a bottle of methylene blue, and a clean razor blade. Methylene blue is a general purpose cationic (positively charged) stain. 2. Locate the Sharps Container. This is where you will dispose of the razor blade when you are finished with this procedure. 3. Place a drop of dH O in the center of the glass slide. Using the razor blade cut a section out of the onion leaf as demonstrated by you instructor 4. 5. Using the forceps, peel the inner epidermis from the onion leaf section leaf and immediately place it into the dH 0. The section should lay flat on the slide. If a fold appears, repeat the step. e 6. Add a small drop of methylene blue to the section. xercise 6: Cells

Explanation / Answer

1. The onion epithelial cells will appear blue in color. Methylene blue is a blue dye that stains negatively charged components of the cells. It will mostly stain the nucleus (center of the cell). The nucleus of onion cells will appear bright blue while the cell wall and cell membrane (edges of the cell) will be stained lighter blue.

2. Onion epidermal cells lack chloroplasts. These cells are buried under the ground away from the light. Hence, they do not carry out photosynthesis. The onion bulb is mostly a storage tissue for energy.

3. The nucleus will be stained an intense blue as it can bind to the methylene blue. DNA is negatively charged which can interact with the positively charged methylene blue to give a blue color.

4. Nucleoli are present in the nucleus. However, it is not possible to distinguish them under low power magnification. They may be visible if using very high magnification of 400X. The nucleoli will be dark intense spots (smaller circles) in the nucleus.

5. The cell cytoplasm occupies the largest volume in an onion cell. It comprises of carbohydrates (Starch), proteins water, salts, other organic molecules, and organelles. It contains a large vacuole that stores water, salts, and organic compounds produced by the plant. The vacuole has different flavor precursors that are present as organic molecules. When cells are damaged, these precursors are cleaves by allinase enzyme to give a scent.

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