cell bio questions Call Bio lon Exchange Chromatography lab questions. Answer th

Question

cell bio questions

Call Bio lon Exchange Chromatography lab questions. Answer the following study questions in your laboratory n otebook or on a separate worksheet. What is the basis for the separation of different compounds by ion ex- change? How ca chromatography? Why are celluloses often used as supports to separate large biologically active proteins? 1. 2. n molecules with the same charge at varying amounts be sepa- rated by 3. What do you think would happen if 0.5M K+ acetate were used first to elute the sample? Why? 4.

Explanation / Answer

1. In Ion Exchange method, the column contain matrix along with Ion Exchanger. If we want to seperate - ve charged molecules from protein mixture, we should use Anion exchanger ( Contain positive chcharge). Vice versa. Here the natural + & - attractions between molecules used for separation

2. By using Affinity or Gel filtration Chromatography

3. Cellulose is a carbohydrate polymer that can form matrix and also form cation exchanger as Corboxy Methyl Cellulose. So it can use extensively in Ion Exchange Chromatography

4. In ion exchange Chromatography, the cationic or positive charged proteins from column elites out. It is due to replacement by K+ ions.

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