Environmental Sample Plate Uninoculated (sterile) Plate (Phage Demo) terde tt1 L

Question

Environmental Sample Plate Uninoculated (sterile) Plate (Phage Demo) terde tt1 Location Sampled Bacteria ONLY Plate Bacteria & Bacteriophage Plate -Booter la NUTRIENT BROTH RESULTS: Both of your nutrient broth tubes should have growth had forgotten to label which tube is which, could you tell what culture is in each tube ? Why or Why Not? (ie: If your neighbor vortexed your tubes by mistake, could you tell which tube contains the pure culture and which one contains the mixed culture?) . If you Dilution Series: How many bacteria per ml were in the original culture tube? Show your calculations. Record results in correct scientific notation. 13

Explanation / Answer

1. Media must be sterile before use. It may be in tubes or petri plates it depends upon the requirenment but media mainly remain sterile in tubes because there is less chance of contamination.

2. There are many method of innoculation but streaking is best method because more the streak less will be colony grow. It will easy to identify the colony clearly.

3. Colony is a group of microorganism or which we have inoculate. The colony is formed by inoculation of microbs in a area that will result in grow of community of them.

4. plaque is a visible structure formed within a cell culture, such as bacterial cultures within some nutrient medium (e.g. agar). The bacteriophage viruses replicate and spread, thus generating regions of cell destructions known as plaques.

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