Spore Staining Twe Methods ae nevessary to destroy endospoeres? In what device a

Question

Spore Staining Twe Methods ae nevessary to destroy endospoeres? In what device are these conditions achieve 2 What is the cofor of endospores after Gram staining? After spore staining? &. What is the secondary stain in the spore stain 9 What is the coloe of the vepetative cell afher the spore stain 10. Ofthese three genera of bacteria, which does not produce endospores: Clostridiam, Mycobacterium, or Bcill ft. Are bacterial endospores reproductive stractures? Explain why or why not. 12. Give three examples of diseases caused by an endospore-forming bacterium and the name of the specific bacterial agent involved.

Explanation / Answer

Question: What conditions are necessary to destroy endospores? in what device are these conditions achieved?

Answer: Heating beyond 100oC under pressurized steam destroys the heat resistant endospores. Autoclave works better because by raising the temperature of steam upto 121oC for atleast 15-20 mins minutes destroys the heat resistant endospores.

Question: What is the color of endospres after gram staining? after spore staining?

Answer: Endospores cannot be stained by gram staining method. The dyes do not penetrate the endospore wall and hence endospres do not takeup the dye of the gram stain.

The endospores can be detected by a specific spore staining method, the endospores appear green in a properly prepared smear.

Question: What is the secondary stain in the spore stain?

Answer: Safranin is the secondary stain in the spore stain. Malachite green is the primary stain and the Safranin is the counterstain that are applied to the smear to stain cell portions in the spore stain method.

Question: what is the color of the vegetative cell after the spore stain?

Answer: In a properly prepared smear, after the spore stain the vegetative cells will appear red or pink in color.  

Question: Of the three genera of bacteria, which does not produce endospores : A. Clostridium, B. Mycobacterium, C. Bacillus

Answer: B. Mycobacterium

Endosporeforming batceria mainly belong to the genera Clostridium and Bacillus.

Question: Are bacterial endospores reproductive structures?

Answer: Bacterial endospores are a resistant, dormant, non-reproductive structure and a survival form of the organism.

Question: Give three examples of diseases caused by an endospore-forming bacterium and the name of the specific bacterial agent involved.

Answer:

1. Anthrax - Bacillus anthracis

2. Tetanus - Clostridium tetani

3.  Botulism - Clostridium botulinum

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