14. (11 points) Your F+ strain has a single auxotrophic mutation (his-), is able

Question

14. (11 points) Your F+ strain has a single auxotrophic mutation (his-), is able to use both lactose and galactose, and is sensitive to erythromycin and resistant to streptomycin. Your F- strain has a single auxotrophic mutation (arg-), is unable to use either lactose or galactose, and is resistant to erythromycin and sensitive to streptomycin Hfr strain A is derived from the F+ strain described above. The order of markers transferred by HfrA is: (first) his str arg gal ery lac (last) a) Your master plate should select for the greater possible number of exconjugants. Assume you are using minimal media (MM). Circle ONE answer for each sentence. The carbon source(s) on the master plate should be Glucose lactose galactose lactose & galactose The master plate should include: erythromycin streptomvcin/ both / neither The master plate should include: argnine histidine both neither

Explanation / Answer

answer-

Glucose is easily metabolized by many bacteria and is usually used as C source in growth media. In minimal media, glucose is thus used to grow mutants.

the master plate should include both antibiotics to distinguish exconjugants on the basis of susceptibility to given drugs.

it should include both arginine and histidine to distinguish exconjugants based on their nutritional needs.

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