Genetics Question 1. An arg cyst his met pro* bacterial strain is known to be ly

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Genetics Question

1. An arg cyst his met pro* bacterial strain is known to be lysogenic for a newly discovered phage that undergoes specialized transduction. However, we'renot sure where, exactly, the phage attachment site might be. The bacterial chromosome map is... met cys arg pro IS The lysogenic strain is used as a source of the phage, and the phages are added to a bacterial strain of genotype ara cys his met pro. Aftera brief incubation, samples of these phage-exposed bacteria are platedon five differenttypes of minimal media (1-5), with the supplementations as indicated in the following table. A plus sign means the minimal media contained that particular supplement (indicated at the top of each column), a minus sign means the media was missing that supplement. The table also shows whethercolonies were observed on each type of plate colonies? no yes no no arg CYS his met pro 2 4 es a) Based on this information, draw and label on this bacterial chromosome map where you'd find the attachment site cys met arg pro his b) How did you arrive at that conclusion? (3 sentences)

Explanation / Answer

1. From the diagram, the attachment site is present between arg and his

2. Leu+ Glu+ and leu- glu-

Some become recombinant, thus having that particular gene.

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